Hibiscus Sabdariffa as Nuclear Stain for Cytological Buccal and Cervical Smear

Abstract

ABSTRACT Introduction: Sudan is one of the biggest countries that produces and exports hibiscus Sabdariffa in Africa. The possibility of HS staining cytological specimens is not well explored. Therefore, the present study aimed to evaluate the staining quality of Hibiscus Sabdariffa on cytological buccal and cervical smear as a cheap viable nuclear stain alternative to hematoxylin. Methods: This study was an experimental descriptive study conducted at Alfajr College for Science and Technology from (June to September 2022). A total of 30 smears, 15 buccal smears collected from students and 15 cervical smears were retrieved from Alfajr's histopathology lab. Results: All (30) slides showed good staining quality with 20% concentration Hibiscus Sabdariffa water extract when mordanted with Iron, and acidified with acetic acid. Conclusion: Hibiscus Sabdariffa is good as nuclear staining for a buccal and cervical pap smear and can replace hematoxylin by adjustment of concentration, time and pH.